Column Chromatography | Protocol
Place a thin layer of sand on top of the silica gel (Figure 1). Using a pipette, wash down any sand that may have stuck to the sides of the column. Drain any additional solvent until the sand is dry, but not down to the silica gel layer. Figure 1. The proper setup for a column chromatography experiment prior to the addition of the sample. 3. Adding the Sample to the Column. Dissolve the sample ...